Vitis

Mariano Puerta was the defending champion but did not compete that year.

Fernando Vicente won in the final 6–4, 7–6^(8–6) against Juan Ignacio Chela.

A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated.

	First round							Second round							Quarterfinals							Semifinals							Final
	1	A Costa	6	64	5
		J A Viloca	2	77	7				J A Viloca	6	6
		N Davydenko	3	4					J A Marín	3	4
		J A Marín	6	6												J A Viloca	6	3	6
	WC	M Hadad	6	3	6										WC	M Hadad	3	6	4
		A Sá	3	6	4			WC	M Hadad	6	6
		R Agénor	64	3				6	M Hantschk	2	2
	6	M Hantschk	77	6																			J A Viloca	610	3
	WC/3	J I Chela	6	6																		WC/3	J I Chela	712	6
		O Stanoytchev	1	1				WC/3	J I Chela	4	77	6
		G Etlis	6	6					G Etlis	6	63	1
		M Charpentier	3	1											WC/3	J I Chela	7	6
	WC	M Tobón	711	6											Q	D Nalbandian	5	3
		E Benfele Álvarez	69	3				WC	M Tobón	67	4
	Q	D Nalbandian	6	6				Q	D Nalbandian	79	6
	8	F Meligeni	2	0																									WC/3	J I Chela	4	66
	5	G Coria	6	7																									2	F Vicente	6	78
		M Russell	4	5				5	G Coria	6	6
		F González	6	6					F González	0	3
		Ó Serrano	4	4											5	G Coria	4	4
	Q	A Simoni	68	6	6										Q	A Simoni	6	6
		D Moyano	710	1	4			Q	A Simoni	6	77
	Q	R Delgado	6	3	7			Q	R Delgado	4	63
	4	N Massú	1	6	5																	Q	A Simoni	3	67
	7	A Stoliarov	6	78																		2	F Vicente	6	79
		P Srichaphan	2	66				7	A Stoliarov	78	4	6
		H Armando	6	6					H Armando	66	6	3
		T Zíb	4	4											7	A Stoliarov	77	3	1
		L Horna	6	61	4										2	F Vicente	64	6	6
	Q	I Hirigoyen	4	77	6			Q	I Hirigoyen	4	64
		M Rodríguez	5	2				2	F Vicente	6	77
	2	F Vicente	7	6

• 2001 Cerveza Club Colombia Open draw
• 2001 Cerveza Club Colombia Open Qualifying draw