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by | Mar 16, 2018 | Hosta

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This article is about Ponceau S. For other uses, see Ponceau (disambiguation).

Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt[1]) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (western blotting), as well as on cellulose acetate membranes.[2] A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on western blots for blot-sequencing.[2] It is also easily reversed with water washes, facilitating subsequent immunological detection.[2] The stain can be completely removed from the protein bands by continued washing.[2] Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.[3]

A nitrocellulose membrane stained with Ponceau S dye for protein detection during western blotting. The blue bands on the left are protein markers for various molecular weights.

See also

References

  1. ^ "Ponceau S solution". Sigma-Aldrich. Retrieved 2016-11-24.
  2. ^ a b c d Al-Amoudi, M. S.; Salman, Mahmoud; Al-Majthoub, M. M.; Adam, Abdel Majid A.; Alshanbari, Naif A.; Refat, Moamen S. (9 October 2013). "Spectral studies to increase the efficiency and stability of laser dyes by charge-transfer reactions for using in solar cells: charge-transfer complexes of Ponceau S with p-chloranil, chloranilic and picric acids". Research on Chemical Intermediates. 41 (5): 3089–3108. doi:10.1007/s11164-013-1417-4. S2CID 96999885.
  3. ^ "Ponceau S solution". Sigma-Aldrich. Retrieved 2016-11-24. The following are two common stain formulations…
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