Hosta

by NG | Mar 16, 2018 | Hosta

How Can We Help?

Pyrrolidonyl-β-naphthylamide (PYR) is a molecule used in microbiology to detect the presence of pyrrolidonyl peptidase.^[1] In the presence of bacteria with pyrrolidonyl peptidase, it is broken down to pyroglutamic acid and 2-naphthylamine. To detect this process, p-dimethylaminocinnamaldehyde is added and a change to a pink color can then be detected.

References

^ Oberhofer, Thomas R. (1986). "Value of the l-pyrrolidonyl-β-naphthylamide hydrolysis test for identification of select gram-positive cocci". Diagnostic Microbiology and Infectious Disease. 4: 43–47. doi:10.1016/0732-8893(86)90055-6.

Techniques in clinical microbiology

Isolation
and culture

Isolation techniques	Asepsis Streak plate Selective media
Cultures by body site	Blood culture Genital culture Sputum culture Throat culture Urine culture Wound culture
Cultures by organism	Bacterial culture Fungal culture Viral culture

Identification
and testing

Manual testing: basic techniques	Colonial morphology Hemolysis Staining Gram stain Acid-fast stain Giemsa stain India ink stain Ziehl–Neelsen stain Wet prep Rapid tests Oxidase Catalase Indole PYR
Manual testing: biochemical and immunologic tests	ALA test Amino acid decarboxylase test Bile solubility test CAMP test Citrate test Coagulase test DNAse test IMViC KOH test Methyl red test Nitrite test ONPG test Oxidative/fermentation glucose test Phenylalanine deaminase test Reverse CAMP test Salt tolerance test Sulfide indole motility test Triple sugar iron test Urease test rapid Voges–Proskauer test X and V factor test Bacitracin susceptibility test Optochin susceptibility test Novobiocin susceptibility test Lancefield grouping RPR test
Automated and point-of-care testing	Analytical profile index MALDI-TOF Polymerase chain reaction VITEK system Rapid strep test Monospot test
Antibiotic susceptibility testing	Beta-lactamase test Disk diffusion test Etest McFarland standards Minimum inhibitory concentration

Equipment

Categories

Table of Contents