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The Makino Botanical Garden (高知県立牧野植物園, Kōchi Kenritsu Makino Shokubutsuen), also known as the Kochi Prefectural Makino Botanical Garden, is a botanical garden located at Godaisan 4200-6, Kōchi, Kōchi Prefecture, Japan. It is open to the public daily except Mondays; an admission fee is charged.

The garden was established in 1958 with a museum dedicated to Tomitaro Makino (1862-1957), the "Father of Japanese Botany", and a research laboratory. Today its collections include Japanese Rhododendron, Acer, Chrysanthemum, serpentine plants, limestone plants, plants of the Sohayaki region, and wild plants of Kōchi Prefecture region.