Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.[2]

The indicator Janus Green B changes colour according to the amount of oxygen present.[3] When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.

References

  1. ^ Janus Green B, at Sigma-Aldrich
  2. ^ Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv für mikroskopische Anatomie 55:558-575(Also Tafel XXXII)
  3. ^ "Archived copy". Archived from the original on 2006-02-24. Retrieved 2007-10-31.{{cite web}}: CS1 maint: archived copy as title (link)