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DescriptionFmicb-10-01490-g002.jpg |
English: Transcription and replication of the vesicular stomatitis virus (VSV) genome. (A) The negative-strand VSV genome in the N–RNA complex serves as a template for transcription (lower) and replication (upper). Le and Tr denote the terminal leader and trailer regions, respectively, in the genome. According to the single-entry, stop-start transcription model, the L–P RdRp complex enters from the 3′-end of the genome and sequentially synthesizes the leader RNA (LeRNA) and five monocistronic mRNAs with a 5′-cap 1 structure and 3′-poly(A) tail (lower). A GDP moiety (red) of GTP, an AMP moiety (blue) of ATP, and two methyl groups (green) are incorporated into the cap 1 structure. The L–P and N0–P (N0: RNA-free N) complexes are required for encapsidation-coupled genome replication (upper). (B) LeRNA is synthesized from the 3′-terminal of the Le promoter in the genome. The conserved gene-start and gene-end sequences serve as internal transcription initiation and termination/polyadenylation signals, respectively. The conserved 5′-terminal mRNA-start sequence acts as a signal for mRNA capping. |
Date | |
Source | https://www.frontiersin.org/articles/10.3389/fmicb.2019.01490/full |
Author | Tomoaki Ogino and Todd J. Green |
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current | 12:56, 16 July 2020 | 1,867 × 1,153 (217 KB) | Guest2625 | Uploaded a work by Tomoaki Ogino and Todd J. Green from https://www.frontiersin.org/articles/10.3389/fmicb.2019.01490/full with UploadWizard |
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