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In enzymology, an arylalkyl acylamidase (EC 3.5.1.76) is an enzyme that catalyzes the chemical reaction

N-acetylarylalkylamine + H2O arylalkylamine + acetate

Thus, the two substrates of this enzyme are N-acetylarylalkylamine and H2O, whereas its two products are arylalkylamine and acetate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acetylarylalkylamine amidohydrolase. This enzyme is also called aralkyl acylamidase.

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