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Ambrose (c. 340 – 4 April 397) was an archbishop of Milan.

Ambrose may also refer to:

People

Ambrose (surname)
Ambrose (given name)
Ambrose (bandleader), or Bert Ambrose (1896–1971), English band-leader and violinist
Ambrose (Cantacuzène) (1947–2009), Russian-Swiss bishop of the Russian Orthodox Church Outside Russia
Ambrose of Alexandria (before 212–c. 250), friend of the Christian theologian Origen
Ambrose of Siena (1220–1286), Italian Dominican teacher, missionary and diplomat

Ambrose University, a private Christian liberal arts college in Calgary, Alberta, Canada
Ambrose Alli University in Ekpoma, Edo State, Nigeria
St Ambrose Barlow Roman Catholic High School, a Catholic technology college in Swinton, Greater Manchester, England, UK
St Ambrose College, a Catholic boys' grammar school in Altrincham, Greater Manchester, England, UK
The Ambrose School, a grade K-12 classical Christian school in Meridian, Idaho, US
St. Ambrose University, Davenport, Iowa, US
St. Ambrose Academy, a Catholic middle school and high school in Madison, Wisconsin, US
Saint Ambrose Catholic School (disambiguation)

Lightship Ambrose, multiple lightships stationed on the Ambrose Channel
Ambrose Light or Ambrose Tower, a light station that replaced the lightships on the Ambrose Channel
Ambrose Medal, an award for service to the Canadian earth science community
Ambrose (golf), a team playing format
Ambrose, Louisiana, a fictional town in House of Wax
"Ambrose (Part 5)", a 1959 novelty song by Linda Laurie
Ambrose Monk, a character from the TV show Monk