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Description5. Метод на MPN (Most probable number) - (Колиметрија на води).ogv |
English: MPN (Most probable number) method:
1. Using a sterile pipette add 1 ml of water in the first five tubes containing LAPV media. 2. Add 1 ml of water, but with ten times higher dilution, in the second tube and 1 ml of water with hundred times higher dilution than the water added in the first five tubes. 3. Incubate the tubes at 37°С, 48 hours. RESULT: A positive result is considered when there is a change in the color of the medium to pink and the presence of gas in the Durham tube. Planned and performed by Sofija Kostandinovska, Department of Biology, FNSM, Ss. Cyril and Methodius University, Skopje, Macedonia.Македонски: Метод на MPN (Most probable number) (Колиметрија на води):
1. Се засејуваат седум епруветки со дурхамови цевчиња, со по 5 мл 5% лактоза андраде пептонска вода, така што во првите 5 се додава по 1 мл директно од примерокот. 2. Втората се засејува, исто така, со 1 мл, но со десет пати поголемо разредување, а третата со 1 мл со стократно поголемо разредување во однос на количеството засеано во првите 5 епрувети. 3. Епруветите се инкубираат на 37°С, за време од 48 часа. РЕЗУЛТАТ: За позитивен резултат се смета ситуацијата каде во епруветката дошло паралелно до промена на бојата на подлогата и продукција на гас (CO2 или H2) која се открива преку насобраниот гас во дурхамовото цевче (најмалку 1:10 од волуменот на цевчето). Осмислено и изведено од Софија Костандиновска, Институт за биологија, ПМФ, УКИМ, Скопје. |
Date | |
Source | Own work |
Author | Deni Ingilizovski |
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:12, 13 October 2021 | 3 min 11 s, 1,920 × 1,080 (171.51 MB) | Dandarmkd (talk | contribs) | Uploaded own work with UploadWizard |
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