How Can We Help?
You are here:
< Back
Size of this preview: 800 × 600 pixels. Other resolutions: 320 × 240 pixels | 640 × 480 pixels | 1,024 × 768 pixels | 1,280 × 960 pixels | 2,560 × 1,920 pixels | 3,072 × 2,304 pixels.
Original file (3,072 × 2,304 pixels, file size: 3.43 MB, MIME type: image/jpeg)
Summary
DescriptionOnion flake. Cells. SEM-BSE.jpg |
Русский: Клетки внутренней поверхности чешуйки лука. Под шагреневой поверхностью клеточной стенки, в толще цитоплазмы, видно ядро и мелкие органеллы. Изображение в обратно-рассеянных электронах (BSE), полученное после контрастирования лантаноидами (BioREE, Россия) на сканирующем электронном микроскопе EVO LS10 (Zeiss, Германия) в режиме низкого вакуума без фиксации, обезвоживания и напыления образца. Такая схема работы с биологическими объектами, разработанная в лаборатории фундаментальных исследований ФГБНУ «НИИ глазных болезней», делает клетки «полупрозрачными» для электронного пучка и позволяет визуализировать на электронном микроскопе их подповерхностный слой, приближая сканирующую электронную микроскопию к оптической. Рассматривание подповерхностного слоя клеток на сканирующем электронном микроскопе при таком способе визуализации очень похоже на рассматривание пузырьков в толще льда на поверхности замерзшего пруда — чем объекты глубже, тем они менее контрастные и более размытые. Соавтор: Суббот А.М. English: Epidermal cells from the inner surface of an onion flake. Beneath the shagreen-like cell walls one can see nuclei and small organelles floating in the cytoplasm. This BSE-image of a lanthanoid-stained sample was taken with EVO LS10 scanning electron microscope (Zeiss, Germany) without prior fixation, nor dehydration, nor sputtering. The unique method of sample preparation used here (BioREE, Russia, with support from the Research Institute of Eye Diseases, Russia) provides an outstanding contrast between subcellular structures giving an impression of light microscopy but at the resolution of SEM. A sample thus prepared resembles a block of ice with air bubbles in it – the deeper an object lies, the lower contrast it has and the more diffused it appears. Coauthor: A.M. Subbot |
Date | |
Source | Own work |
Author | Ivan A. Novikov |
Licensing
I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution-Share Alike 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.
This image was uploaded as part of European Science Photo Competition 2015. |
Items portrayed in this file
depicts
creator
some value
Wikimedia username: Novikov76
author name string: Ivan A. Novikov
copyright status
copyrighted
copyright license
Creative Commons Attribution-ShareAlike 4.0 International
inception
14 October 2015
source of file
original creation by uploader
instance of
scanning electron micrograph
fabrication method
scanning electron microscopy
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 11:55, 11 November 2015 | 3,072 × 2,304 (3.43 MB) | Novikov76 | User created page with UploadWizard |
File usage
The following pages on the English Wikipedia use this file (pages on other projects are not listed):
Global file usage
The following other wikis use this file:
- Usage on gl.wikipedia.org
- Usage on id.wikipedia.org
- Usage on zh.wikipedia.org
Categories
-
Annuals36
-
Bulbs, Corms & Tubers41
-
Ferns27
-
Fruits3
-
Garden Plants23
-
Grasses26
-
Herb17
-
Insects1
-
Mammals1
-
Midwest Native Plants0
-
Northeast Native Plants112
-
Perennials123
-
Rose1
-
Shrubs47
-
Trees112
-
Tropical Plants53
-
Upland Birds5
-
Vines18
-
Viola Tricolor1
-
Water Gardening & Plants9
-
Waterfowl0
-
Wetland Birds0
-
Wetland Plants4
-
Wildbirds172
-
Wildflowers1
-
Woodland Plants29
Table of Contents
Recent Comments